anti α4β7  (R&D Systems)

Journal: Immunology letters

Article Title: Identification of S100A9 as a target for diagnosis and treatment of Crohn's Disease after Vedolizumab treatment failure.

doi: 10.1016/j.imlet.2025.107027

Figure Lengend Snippet: Fig. 7. Anti-α4β7 integrin antibody combined with inhibitor of S100A9 reversed DSS induced colon injury. (A) Comparison of changes in the body weight among DSS group, Paquinimod group, BE0034 group and Combination group. The changes of body weight were expressed as a percentage of the original weight at the start of the experiments during a period of 7-day observation. *p < 0.05, **p < 0.01, ***p < 0.001. (B) Comparison of the disease activity index among DSS group, Paquinimod group, BE0034 group and Combination group. The disease activity index (DAI) was measured on day7; DAI = weight loss score + stool characters score + hematochezia score. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. (C) Colon length among DSS group, Paquinimod group, BE0034 group and Combination group. (D) Gross macroscopic architecture of colonic tissues. (E and F) H&E staining analysis of colon tissues.

Article Snippet: Anti-α4β7 integrin antibody (Bio X Cell, BE0034) and corresponding isotype control antibody (Bio X Cell, BE0089) was selected at 200 μg/mouse and injected into the tail vein on day2, day4 and day6.

Techniques: Comparison, Activity Assay, Staining

Journal: Immunity

Article Title: CRISPR screens unveil nutrient-dependent lysosomal and mitochondrial nodes impacting intestinal tissue-resident memory CD8 + T cell formation

doi: 10.1016/j.immuni.2024.09.013

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: anti-α4β7 (DATK32), PE , Thermo Fisher Scientific , Cat# 12-5887-82; RRID: AB_657803.

Techniques: Purification, Virus, Expressing, Mutagenesis, Recombinant, Electron Microscopy, Control, Modification, Plasmid Preparation, Cell Isolation, Transfection, Sample Prep, Reverse Transcription, SYBR Green Assay, Microarray, RNA Sequencing, Knock-In, Sequencing, Software, Flow Cytometry, Microscopy, Real-time Polymerase Chain Reaction

Journal: Immunity

Article Title: CRISPR screens unveil nutrient-dependent lysosomal and mitochondrial nodes impacting intestinal tissue-resident memory CD8 + T cell formation

doi: 10.1016/j.immuni.2024.09.013

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: For analysis of surface markers, cells were stained in PBS containing 2% FBS for 30 min at room temperature with the appropriate surface antibodies: anti-TCRβ (H57-597, Biolegend), anti-CD8α (53-6.7, Cytek/Tonbo), anti-CD103 (2E7, Thermo Fisher Scientific), anti-CD69 (H1.2F3, Biolegend), anti-CCR9 (CW-1.2, BD Bioscience), anti-CD45.1 (A20, Biolegend), anti-CD45.2 (104, Biolegend), anti-Thy1.1 (OX-7, Biolegend), anti-Thy1.2 (53-2.1, Biolegend), streptavidin (Thermo Fisher Scientific), anti-α4β7 (DATK32, Thermo Fisher Scientific), anti-KLRG1 (2F1, Biolegend), anti-CD62L (MEL-14, Biolegend) and fixable viability dye (catalog #65-0865, Thermo Fisher Scientific).

Techniques: Purification, Virus, Expressing, Mutagenesis, Recombinant, Electron Microscopy, Control, Modification, Plasmid Preparation, Cell Isolation, Transfection, Sample Prep, Reverse Transcription, SYBR Green Assay, Microarray, RNA Sequencing, Knock-In, Sequencing, Software, Flow Cytometry, Microscopy, Real-time Polymerase Chain Reaction